PMC

(a) Reconstructed image with the ideal situation and no geometric calibration. (b) axis tilt = 1 degree; (c) axis tilt = 2 degrees; (d) axis tilt = 3 degrees; (e) axis tilt = 5 degrees. (f) Reconstructed image with the ideal situation and geometric calibration. (g) Reconstructed image of (b) after calibration. (h) Reconstructed image of (c) after calibration. (i) Reconstructed image of (d) after calibration. (j) Reconstructed image of (e) after calibration.

(a) Reconstructed image with the ideal situation and no geometric calibration. (b) axis shift = 1 mm; (c) axis shift = 3 mm; (d) axis shift = 5 mm; (e) axis shift = 10 mm. (f) Reconstructed image with the ideal situation and geometric calibration. (g) Reconstructed image of (b) after calibration. (h) Reconstructed image of (c) after calibration. (i) Reconstructed image of (d) after calibration. (j) Reconstructed image of (e) after calibration.

Inter-rater reliability for each method, on log count scale, and therefore showing relative differences. (a) Earle and Perez graph. X axis: Mean (geometric) count across raters for each sample. Y axis: Relative difference between each raters count and the geometric mean of all counts from the same sample. (b) Thick film method. X axis: Mean (geometric) count across raters for each sample. Y axis: Relative difference between each raters count and the geometric mean of all counts from the same sample. (c) Thick film method restricted to EDTA Blood. X axis: Mean (geometric) count across raters for each sample. Y axis: Relative difference between each raters count and the geometric mean of all counts from the same sample.

Bland and Altman plots to demonstrate the presence of any bias between different methods of counting the parasite loads, on a log scale, to show relative differences. (a) Comparison of Earle and Perez with thick film methods (after averaging each method across all raters). X axis: Mean (geometric) averaged across both methods. Y axis: Relative difference between mean (geometric) counts from each method (Earle and Perez over Thick film method). (b Comparison of Earle and Perez with Thick Film methods (after averaging Earle and Perez across all raters). X axis: Mean (geometric) averaged across both methods. Y axis: Relative difference between mean (geometric) counts from each method (Earle and Perez over Thick film method). (c) Comparison of Thick Film with Thin Film methods (after averaging thick Film method across all raters). X axis: Mean (geometric) averaged across both methods. Y axis: Relative difference between mean (geometric) counts from each method (Thick film over thin film method).